Composite
AtLCD

Part:BBa_K3665009:Design

Designed by: Wu Yanjun   Group: iGEM20_JACOXH_China   (2020-10-25)


AtLCD, a metalloprotein its expression regulated by RBS4, RBS5, OR RBS52


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 849


Design Notes

Codon biax analysis was used to adapt to the expression in Bacillus subtilis.


Source

Composite

References